Faculty Name: Dr. Gunjan Mehta
Course Name: Advanced Fluorescence Microscopy and Image Processing
Course Period: 29 th Jul to 18 th Oct 2024 (Monday 4: 00 PM to 05:30 PM; Thursday 2:30 PM to 04:00 PM)
Number of Credits: 2 credits
Course Contents:
Introduction to fluorescence microscopy and its applications; design of a fluorescence microscope and its optics; various illumination strategies such as wide field, confocal, TIRF/HILO, light sheet; fluorescent proteins, dyes, and fluorescence labeling strategies; 3D imaging; live cell imaging; time-lapse imaging; super-resolution microscopy (SIM, STED, STORM/PALM); single-molecule imaging; optical tweezers and traction force microscopy; high content imaging; techniques such as FRET, FRAP, FLIM, immunofluorescence, Fluorescence In-situ Hybridization (FISH), Spatial mapping of gene expression (RNAscope); digital images and camera technologies; introduction to Fiji/ImageJ, application of artificial intelligence/machine learning and virtual reality in image analysis.
The practical component of this course includes image processing, data analysis, quantification, and visualization using Fiji/ImageJ. Quantification of biological information from the microscopic images, intensity measurement, image segmentation, colocalization, quantification, and visualization of 3D images, deconvolution, 3D rendering, and reconstruction.
What you'll learn: Students will learn basics of fluorescence microscopy and its applications in life science/biology research. Different illumination strategies such as wide-field, confocal, light- sheet, TIRF, two-photon and various techniques such as FRET, FRAP, FLIM, immunofluorescence, 5D imaging, time-lapse imaging, single-molecule and super-resolution imaging etc. will be taught. Students will also learn basics of digital images and camera technologies used in fluorescence microscopy and how to quantify biological information from the digital images using Fiji/ImageJ.
About the Instructor: Dr. Gunjan Mehta is an assistant professor in the Department of Biotechnology at IIT Hyderabad. He used advanced imaging technologies to study cell division during his Ph.D. (2009-2015) at IIT Bombay. He worked as a Post-Doctoral Fellow (2015-2020) at the National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, USA. During his post-doc, he developed a single-molecule imaging technique for quantifying the dynamics of proteins in live cells at the NCI optical microscopy core facility.
He has more than 13 years of experience developing and applying fluorescence microscopy techniques for biomedical research. Currently, his research group at IIT Hyderabad studies the mechanism of chromosome dynamics and gene regulation using single-molecule imaging approaches.
